研究成果

研究推進体発足以降（2016年６月〜）

学術雑誌への研究論文発表

(* corresponding author (last authorではない場合のみ表記))

原著論文

Masaya Igase, Yuki Nemoto, Kazuhito Itamoto, Kenji Tani, Munekazu Nakaichi, Masashi Sakurai, Yusuke Sakai, Shunsuke Noguchi, Masahiro Kato, Toshihiro Tsukui & Takuya Mizuno. (2020)

A pilot clinical study of the therapeutic antibody against canine PD-1 for advanced spontaneous cancers in dogs.

Sci Rep. (in press)

Fujiwara N, Shibutani S, Sakai Y, Watanabe T, Kitabayashi I, Oshima H, Oshima M, Hoshida H, Akada R, Usui T, Ohama T*, Sato K. (2020)

Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6.

Cancer Sci. (in press)

Ikeda S, Tsuji S, Ohama T*, Sato K. (2020)

Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.

J Biochem. (in press)

Nakaichi M, Iseri T, Horikirizono H, Sakai Y, Itoh H, Sunahara H, Itamoto K, Tani K. (2020)

Clinical features and their course of pituitary carcinoma with distant metastasis in a dog.

J Vet Med Sci. (in press)

Sakai O, Igase M, Mizuno T. (2020)

Optimization of canine CD20 chimeric antigen receptor T cell manufacturing and in vitro cytotoxic activity against B-cell lymphoma.

Vet Comp Oncol. (in press)

Sakai O, Ii T, Uchida K, Igase M, Mizuno T. (2020)

Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha.

Monoclon Antib Immunodiagn Immunother. 39(4):129-134.

Hamamura Y, Nakagawa T, Okuda M, Noguchi S, Igase M, Mizuno T. (2020)

The inhibitory effect of canine interferon gamma on the growth of canine tumors.

Res Vet Sci. 132:466-473.

Mizuno T, Kato Y, Kaneko MK, Sakai Y, Shiga T, Kato M, Tsukui T, Takemoto H, Tokimasa A, Baba K, Nemoto Y, Sakai O, Igase M. (2020)

Generation of a canine anti-canine CD20 antibody for canine lymphoma treatment.

Sci Rep. 10(1):11476.

Fujimori D, Kinoshita J, Yamaguchi T, Nakamura Y, Gunjigake K, Ohama T, Sato K, Yamamoto M, Tsukamoto T, Nomura S, Ohta T, Fushida S. (2020)

Established fibrous peritoneal metastasis in an immunocompetent mouse model similar to clinical immune microenvironment of gastric cancer.

BMC Cancer. 20(1):1014.

Igase M, Morinaga Y, Kato M, Tsukui T, Sakai Y, Okuda M, Mizuno T. (2020)

Establishment of rat anti-canine DEP domain containing 1B (DEPDC1B) monoclonal antibodies.

J Vet Med Sci. 82(4):483-487.

Igase M, Fujiki N, Shibutani S, Sakai H, Noguchi S, Nemoto Y, Mizuno T. (2020)

Tenovin-6 induces the SIRT-independent cell growth suppression and blocks autophagy flux in canine hemangiosarcoma cell lines.

Exp Cell Res. 388(1):111810.

Itoh H, Nishikawa S, Tani K, Sunahara H, Nakaichi M, Iseri T, Taura Y, Itamoto K. (2020)

Cystine transporter expression is a marker to identify a subpopulation of canine adipose-derived stem cells.

J Vet Med Sci. 2020 Jun 16;82(6):713-720.

Nakashima T, Kawazoe Y, Iseri T, Miyamoto K, Fujimoto Y, Kato S. (2020)

The effect of positive-end-expiratory pressure on stroke volume variation: An experimental study in dogs.

Clin Exp Pharmacol Physiol. 2020 Jun;47(6):1014-1019.

Goshima T, Habara M, Maeda K, Hanaki S, Kato Y, Shimada M. (2019)

Calcineurin regulates cyclin D1 stability through dephosphorylation at T286.

Sci Rep. 9(1):12779.

Nishimura K, Johmura Y, Deguchi K, Jiang Z, Uchida KSK, Suzuki N, Shimada M, Chiba Y, Hirota T, Yoshimura SH, Kono K, Nakanishi M. (2019)

Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase.

Nat Commun. 10(1):981.

Yamanaka K, Okuda M, Mizuno T. (2019)

Functional characterization of canine wild type glucocorticoid receptor and an insertional mutation in a dog.

BMC Vet Res. 15(1):363.

Igase M, Shibutani S, Kurogouchi Y, Fujiki N, Hwang CC, Coffey M, Noguchi S, Nemoto Y, Mizuno T. (2019)

Combination Therapy with Reovirus and ATM Inhibitor Enhances Cell Death and Virus Replication in Canine Melanoma.
Mol Ther Oncolytics. 15:49-59.

Kobayashi K, Baba K, Igase M, Primarizky H, Nemoto Y, Shimokawa Miyama T, Kambayashi S, Mizuno T, Okuda M. (2019)

Tissue factor procoagulant activity in the tumor cell lines and plasma of dogs with various malignant tumors.

J Vet Med Sci. 81(12):1713-1721.

Amaha T, Ishikawa C, Ishigaki K, Horikirizono H, Iizuka K, Nagumo T, Tamura K, Seki M, Edamura K, Asano K. (2019)

Computed tomographic-derived measurements of shunt fraction and hepatic perfusion in dogs with a single extrahepatic portosystemic shunt in a clinical setting.

J Am Vet Med Assoc. 255(7):821-827.

Horikirizono H, Ishigaki K, Amaha T, Iizuka K, Nagumo T, Tamura K, Seki M,
Edamura K, Watari T, Asano K. (2019)

Clinical features and surgical treatment of
inflammatory colorectal polyps in miniature dachshunds: 40 cases
(2002-2015).

Jpn J Vet Res. 67(2), 173-183.

Horikirizono H, Ishigaki K, Amaha T, Iizuka K, Nagumo T, Tamura K, Seki M, Edamura K, Asano K. (2019)

Inhibition of growth of canine-derived vascular endothelial cells by non-steroidal anti-inflammatory drugs and atrial natriuretic peptide.

J Vet Med Sci. 2019 May 31;81(5):776-779.

Sakurai M, Kurachi J, Sakai Y, Morimoto M. (2019)

Nippostrongylus brasiliensis infection inhibits hippocampal neurogenesis in mice.

Neuropathology. 2019 Dec;39(6):425-433.

Kanai Y, Kawagishi T, Sakai Y, Nouda R, Shimojima M, Saijo M, Matsuura Y, Kobayashi T. (2019)

Cell-cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses.

PLoS Pathog. 15(4):e1007675.

Sakai Y*, Hiyama M, Kagimoto S, Mitsui Y, Imaiumi M, Okayama T, Haradono K, Sakurai M, Morimoto M. (2019)

Enteric peripheral neuroblastoma in a calf.

J Vet Med Sci. 81(6):824-827.

Khatun A, Sakurai M, Sakai Y, Tachibana M, Ohara N, Morimoto M. (2019)

Mycobacterial infection induces eosinophilia and production of α-defensin by eosinophils in mice.

J Vet Med Sci. 81(1):138-142.

Umata K, Sakai Y, Ikeda S, Tsuji S, Kawasaki H, Ohama T*, Sato K. (2019)

Distribution of SET/I2PP2A protein in gastrointestinal tissues.

PLoS One. 14(9):e0222845.

Tsuji S, Ohama T, Nakagawa T, Sato K. (2019)

Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma.

J Vet Med Sci. doi: 10.1292/jvms.19-0311.

Hwang CC, Igase M, Okuda M, Coffey M, Noguchi S, Mizuno T. (2019)

Reovirus changes the expression of anti-apoptotic and proapoptotic proteins with the c-kit downregulation in canine mast cell tumor cell lines.

Biochem Biophys Res Commun. 517(2):233-237.

Igase M, Shousu K, Fujiki N, Sakurai M, Bonkobara M, Hwang CC, Coffey M, Noguchi S, Nemoto Y, Mizuno T. (2019)

Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells.

Vet Comp Oncol. 17(2):184-193.

Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Fukui M, Harada H, Mizuno T, Sakai Y, Takasu M, Kaneko MK, Kato Y. (2019)

PMab-210: A Monoclonal Antibody Against Pig Podoplanin.

Monoclon Antib Immunodiagn Immunother. 38(1):30-36.

Tsuruta Y, Shibutani S, Watanabe R, Iwata H. (2019)

Apoptosis induced by Ibaraki virus does not affect virus replication and cell death in hamster lung HmLu-1 cells.

J Vet Med Sci. 81(2):197-203.

Onishi K, Shibutani S, Goto N, Maeda Y, Iwata H. (2019)

Amino acid starvation accelerates replication of Ibaraki virus.

Virus Res. 260:94-101.

Yamada S, Itai S, Furusawa Y, Sano M, Nakamura T, Yanaka M, Handa S, Hisamatsu K, Nakamura Y, Fukui M, Harada H, Mizuno T, Sakai Y, Ogasawara S, Murata T, Uchida H, Tahara H, Kaneko MK, Kato Y. (2018)

Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52.

Monoclon Antib Immunodiagn Immunother. 37(5):224-228.

Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Handa S, Mizuno T, Maeda K, Fukui M, Harada H, Kaneko MK, Kato Y. (2018)

Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin.

Monoclon Antib Immunodiagn Immunother. 37(5):233-237.

Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52.

Monoclon Antib Immunodiagn Immunother. 37(5):224-228.

Kato Y, Mizuno T, Yamada S, Nakamura T, Itai S, Yanaka M, Sano M, Kaneko MK.

Establishment of P38Bf, a Core-Fucose-Deficient Mouse-Canine Chimeric Antibody Against Dog Podoplanin.

Monoclon Antib Immunodiagn Immunother. 37(5):218-223.

Yabe R, Tsuji S, Mochida S, Ikehara T, Usui T, Ohama T*, Sato K. (2018)

A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation.

FEBS Open Bio. 8(9):1486-1496.

Nemoto Y, Shosu K, Okuda M, Noguchi S, Mizuno T. (2018)

Development and characterization of monoclonal antibodies against canine PD-1 and PD-L1.

Vet Immunol Immunopathol. 198:19-25.

Usui T*, Sakurai M, Umata K, Elbadawy M, Ohama T, Yamawaki H, Hazama S, Takenouchi H, Nakajima M, Tsunedomi R, Suzuki N, Nagano H, Sato K, Kaneda M, Sasaki K. (2018)

Hedgehog Signals Mediate Anti-Cancer Drug Resistance in Three-Dimensional Primary Colorectal Cancer Organoid Culture.

Int J Mol Sci. 6;19(4).

Usui T, Sakurai M, Umata K, Yamawaki H, Ohama T, Sato K. (2018)

Preparation of Human Primary Colon Tissue-Derived Organoid Using Air Liquid Interface Culture.

Curr Protoc Toxicol. 75:22.6.1-22.6.7.

Enjoji S, Yabe R, Tsuji S, Yoshimura K, Kawasaki H, Sakurai M, Sakai Y, Takenouchi H, Yoshino S, Hazama S, Nagano H, Oshima H, Oshima M, Vitek MP, Matsuura T, Hippo Y, Usui T, Ohama T*, Sato K. (2018)

Stemness is Enhanced in Gastric Cancer by a SET/PP2A/E2F1 Axis.

Mol Cancer Res. 16(3):554-563

Kanai Y, Kawagishi T, Okamoto M, Sakai Y, Matsuura Y, Kobayashi T. (2018)

Lethal murine infection model for human respiratory disease-associated Pteropine orthoreovirus.

Virology. 514:57-65.

Noguchi S, Shibutani S, Fukushima K, Mori T, Igase M, Mizuno T. (2018)

Bosutinib, an SRC inhibitor, induces caspase-independent cell death associated with permeabilization of lysosomal membranes in melanoma cells.

Vet Comp Oncol. Mar 28.

Hirano J, Okamoto T, Sugiyama Y, Suzuki T, Kusakabe S, Tokunaga M, Fukuhara T, Sasai M, Tougan T, Matsunaga Y, Yamashita K, Sakai Y, Yamamoto M, Horii T, Standley DM, Moriishi K, Moriya K, Koike K, Matsuura Y. (2017)

Characterization of SPP inhibitors suppressing propagation of HCV and protozoa.

Proc Natl Acad Sci U S A. 114(50):E10782-E10791.

Sakai Y, Kawachi K, Terada Y, Omori H, Matsuura Y, Kamitani W. (2017)

Two-amino acids change in the nsp4 of SARS coronavirus abolishes viral replication.

Virology. 510:165-174.

Shibutani S*, Okazaki H, Iwata H (2017)

Dynamin-dependent amino acid endocytosis activates mechanistic target of rapamycin complex 1 (mTORC1)

Journal of Biological Chemistry 292(44):18052-18061

Usui T*, Sakurai M, Nishikawa S, Umata K, Nemoto Y, Haraguchi T, Itamoto K, Mizuno T, Noguchi S, Mori T, Iwai S, Nakagawa T, Yamawaki H, Ohama T, Sato K. (2017)

Establishment of a dog primary prostate cancer organoid using the urine cancer stem cells.

Cancer Sci. 108(12):2383-2392.

Kawano K, Mizuno T. (2017)

A pilot study of the effect of pullulan-conjugated Der f 2 allergen-specific immunotherapy on canine atopic dermatitis.

Vet Dermatol. 28(6):583-e141.

Hara K, Iio A, Asahina R, Takahashi M, Mori T, Nishida H, Kamishina H, Sakai H, Kitoh K, Mizuno T, Tsujimoto H, Maeda S. (2017)

Characterization of a novel canine T-cell line established from a dog with cutaneous T-cell lymphoma.

J Dermatol Sci. 88(2):254-256.

Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Eto S, Hiyama M, Iseri T, Itoh Y, Nakaichi M, Sakai Y, Tani K, Taura Y, Itamoto K. (2017)

Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential.

World J Stem Cells. 9(10):179-186.

Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Hiyama M, Iseri T, Itoh Y, Nakaichi M, Taura Y, Tani K, Itamoto K. (2017)

Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells.

J Vet Med. 2017:5701016.

Haraguchi T, Kimura S, Itoh H, Nishikawa S, Hiyama M, Tani K, Iseri T, Itoh Y, Nakaichi M, Taura Y, Itamoto K. (2017)

Comparison of postoperative pain and inflammation reaction in dogs undergoing preventive laparoscopic-assisted and incisional gastropexy.

J Vet Med Sci. 79(9):1524-1531.

Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Hiyama M, Iseri T, Itoh Y, Nakaichi M, Taura Y, Tani K, Itamoto K. (2017)

Identification of rhodamine 123-positive stem cell subpopulations in canine hepatocellular carcinoma cells.

Biomed Rep. 7(1):73-78.

Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Hiyama M, Eto S, Iseri T, Itoh Y, Tani K, Nakaichi M, Taura Y, Itamoto K. (2017)

Aldehyde dehydrogenase activity identifies a subpopulation of canine adipose-derived stem cells with higher differentiation potential.

J Vet Med Sci. 79(9):1540-1544.

Kake S, Tsuji S, Enjoji S, Hanasaki S, Hayase H, Yabe R, Tanaka Y, Nakagawa T, Liu HP, Chang SC, Usui T, Ohama T*, Sato K (2017)

The Role of SET/I2PP2A in canine mammary tumors

Scientific Reports. 7(1):4279（doi:10.1038/s41598-017-04291-7.）

Usui T*, Sakurai M, Kawasaki H, Ohama T, Yamawaki H, Sato K. (2017)

Establishment of a novel three-dimensional primary culture model for hippocampal neurogenesis

Physiological Reports, 5(12).

Kawasaki H, Saotome T, Usui T, Ohama T, Sato K (2017)

Regulation of intestinal myofibroblasts by KRas-mutated colorectal cancer cells through heparin-binding epidermal growth factor-like growth factor

Oncoogyl Report, 37(5): 3128-3136（doi: 10.3892/or.2017.5520.)

Kake S, Usui T*, Ohama T, Yamawaki H, Sato K (2017)

Death-associated protein kinase 3 controls tumor progression of A549 cells through ERK MAPK/c-Myc signaling

Oncology Report, 37(2): 1100-1106 (doi: 10.3892/or.2017.5359.)

Sakai S, Sakurai M, Sakai H, Kubo M, Hiraoka H, Baba K, Okuda M, Mizuno T. (2017)

Molecular cloning of canine Wilms’ Tumor 1 for immunohistochemical analysis in canine tissues.

J Vet Med Sci. Jul 28;79(7):1272-1277. (doi: 10.1292/jvms.17-0229.)

Nemoto Y, Haraguchi T, Shimokawa Miyama T, Kobayashi K, Hama K, Kurogouchi Y, Fujiki N, Baba K, Okuda M, Mizuno T. (2017)

Pancreatic abscess in a cat due to Staphylococcus aureus infection.

J Vet Med Sci. doi: 10.1292/jvms.17-0026.

Itoh H, Itamoto K, Eto S, Haraguchi T, Nishikawa S, Tani K, Itoh Y, Hiyama M, Iseri T, Nakaichi M, Taura Y. (2017)

Craniocervical junction abnormalities with atlantoaxial subluxation caused by ventral subluxation of C2 in a dog.

Open Vet J. 7(1):65-69.

Iwata T, Uchino T, Koyama A, Johmura Y, Koyama K, Saito T, Ishiguro S, Arikawa T, Komatsu S, Miyachi M, Sano T, Nakanishi M and Shimada M. (2017)

The G2 checkpoint inhibitor CBP-93872 increases the sensitivity of colorectal and pancreatic cancer cells to chemotherapy.

PLOS ONE, 12: e0178221

Usui T*, Sakurai M, Enjoji S, Kawasaki H, Umata K, Ohama T, Fujiwara N, Yabe R, Tsuji S, Yamawaki H, Hazama S, Takenouchi H, Nakajima M, Tsunedomi R, Suzuki N, Nagano H, Sato K. (2016)

Establishment of a novel model for anti-cancer drug resistance in three-dimensional primary culture of tumor microenvironment.

Stem Cells International, 7053872 (doi: 10.1155/2016/7053872.)

Kobayashi K, Shimokawa Miyama T, Itamoto K, Noguchi S, Baba K, Mizuno T, Okuda M. (2016)

Development of hepatocellular carcinoma after long-term immunosuppressive therapy including danazol in a dog.

J Vet Med Sci. 78: 1611-1614.

Roode SC, Rotroff D, Richards KL, Moore P, Motsinger-Reif A, Okamura Y, Mizuno T, Tsujimoto H, Suter SE, Breen M. (2016)

Comprehensive genomic characterization of five canine lymphoid tumor cell lines.

BMC Vet Res. 17: 207.

Kawano K, Shimakura H, Nagata N, Masashi Y, Suto A, Suto Y, Uto S, Ueno H, Hasegawa T, Ushigusa T, Nagai T, Arawatari Y, Miyaji K, Ohmori K, Mizuno T. (2016)

Prevalence of food-responsive enteropathy among dogs with chronic enteropathy in Japan.

J Vet Med Sci. 78: 1377-1380.

Ema Y, Igase M, Takeda Y, Yanase T, Umeki S, Hiraoka H, Okuda M, Mizuno T. (2016)

Investigation of the cytotoxic effect of flavopiridol in canine lymphoma cell lines.

Vet Comp Oncol. Suppl 1: 95-106.

Hwang CC, Umeki S, Igase M, Coffey M, Noguchi S, Okuda M, Mizuno T. (2016)

The effects of oncolytic reovirus in canine lymphoma cell lines.

Vet Comp Oncol. Suppl 1: 61-73.

Johmura Y, Yamashita E, Shimada M, Nakanishi K and Nakanishi M. (2016)

Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation.

Scientific Report, 6: 31194.

Shimada M*, Goshima T, Matsuo H, Johmura Y, Haruta M, Murata K, Tanaka H, Ikawa M, Nakanishi K and Nakanishi M. (2016)

Essential role of auto-activation circuitry on Aurora B-mediated H2AX-pS121 in mitosis.

Nature Commun., 7: 12059.

Sharif J, Endo A. T, Nakayama M, Karimi M. M, Shimada M, Katsuyama K, Goyal P, Brind'Amour J, Sun M, Sun Z, Ishikura T, Mizutani-Koseki Y, Ohara O, Shinkai Y, Nakanishi M, Xie H, Lorincz C. M and Koseki H. (2016)

Activation of Endogenous Retroviruses in Dnmt1–/– ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA.

Cell Stem Cell, 19: 81-94.

総説

Ohama T (2018)

The multiple functions of protein phosphatase 6

Biochim Biophys Acta. doi: 10.1016/j.bbamcr.2018.07.015. [Epub ahead of print]

円城寺秀平、大浜剛（2017）

炎症・がんにおけるタンパク質脱リン酸化酵素PP2Aの役割

日薬理誌, 149. 208-212

Shimada M and Nakanishi M (2016)

Aurora B twists on histones for activation

Cell Cycle, 15: 3321.

Klionsky DJ, Shibutani S et al.

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Autophagy. 2016; 12(1): 1-222.

国際学会又は全国大会での発表

臼井達也、大浜剛、佐藤晃一

Establishment of a novel model for anti-cancer drug resistance using colorectal cancer organoid第90回日本薬理学会年会　長崎ブリックホール・長崎新聞文化ホール

2017年3月15日（口頭発表）

円城寺秀平、矢部滝太郎、吉村和大、川崎秀吉、櫻井優、坂井祐介、竹之内寛子、吉野茂文、硲彰一、永野浩昭、大島浩子、大島正伸、Michael P. Vitek、松浦哲也、筆宝義隆、臼井達哉、大浜剛、佐藤晃一

PP2A阻害因子SET/I2PP2Aの胃癌における役割の解明

第90回日本薬理学会年会　長崎ブリックホール・長崎新聞文化ホール

2017年3月15日（口頭発表）

辻俊也、加計悟、円城寺秀平、花崎さやか、早瀬寛志、矢部滝太郎、田中由依子、中川貴之、臼井達也、大浜剛、佐藤晃一

イヌ乳腺腫瘍におけるSET/I2PP2Aの役割

第90回日本薬理学会年会　長崎ブリックホール・長崎新聞文化ホール

2017年3月15日（口頭発表）

矢部滝太郎、三浦茜、臼井達哉、Ingrid Mudrak、Egon Ogris、大浜剛、佐藤晃一

PP2A特異的脱メチル化酵素PME-1はPP2Aをユビキチン・プロテアソーム系分解から保護する

第39回日本分子生物学会　パシフィコ横浜

2016年12月2日（ポスター発表）

川崎秀吉，馬田康司，硲彰一，鈴木伸明，永野浩昭，臼井達哉，大浜剛，佐藤晃一

Sphingosine-1-Phosphateは結腸癌微小環境において筋線維芽細胞の遊走を制御する

第39回日本分子生物学会　パシフィコ横浜

2016年12月2日（ポスター発表）

臼井達哉、櫻井優、円城寺秀平、川崎秀吉、馬田浩司、佐野昭子、中島正夫、恒富亮一、硲彰一、大浜剛、佐藤晃一

Establishment of a novel model for anti-cancer drug resistance in three-dimensional primary culture of tumor microenvironment

第39回日本分子生物学会　パシフィコ横浜

2016年12月1日（ポスター発表）

PP2A阻害因子SET/I2PP2Aの胃癌における役割の解明

第39回日本分子生物学会　パシフィコ横浜

2016年12月1日（ポスター発表）

藤原信行、臼井達哉、大浜剛、佐藤晃一

PP2AとDAPK3はBeclin 1のリン酸化を調節しオートファジーを制御する

第39回日本分子生物学会　パシフィコ横浜

2016年12月1日（ポスター発表）

Yabe R, Usui T, Sato K, Ohama T

Modification of PP2A Methylation Status Assay and Implication for Protein Phosphatase Methylesterase-1 (PME-1) as a Therapeutic Target for a Subset of Melanoma

The 12th International Conference on Protein Phosphatase, Osaka, Japan

2016年10月29日（ポスター発表・査読なし）奨励賞受賞演題

Enjoji S, Yabe R, Yoshimura K, Kawasaki H, Sakurai M, Sakai Y, Takenouchi H, Yoshino S, Hazama S, Nagano H, Ohshima H, Ohshima M, Vitek MP, Matsuura T, Hippo Y, Ohama T, Sato K

SET/I2PP2A Is a Prognostic Marker and a Potential Therapeutic Target for Gastric Cancer

The 12th International Conference on Protein Phosphatase, Osaka, Japan

2016年10月29日（ポスター発表・査読なし）

Fujiwara N, Ohama T, Sato K

Regulation of Beclin 1 Phosphorylation and Autophagy by PP2A and DAPK3

The 12th International Conference on Protein Phosphatase, Osaka, Japan

2016年10月29日（ポスター発表・査読なし）

Hwang CC, Coffery M, Noguchi S, Mizuno T.

Reovirus degraes c-kit and dysregulates the ras signaling pathway to induce apoptotic cell death in canine mast cell tumor (MCT).

Veterinary Cancer Society Annual Meeting (Orlando, Florida) 2016.10.22

Igase M, Hwang CC, Sakurai M, Shimokawa T, Haraguchi T, Tani K, Itamoto K, Nakaichi M, Noguchi S, Mizuno T. Safety and prrof-of-concept evaluation of reovirus as oncolytic virotherapy in dogs with spontaneously ocurring tumors. Veterinary Cancer Society Annual Meeting (Orlando, Florida) 2016.10.22

伊賀瀬雅也、Chung C. Hwang, 野口俊介、水野拓也.

犬の腫瘍症例に対するレオウイルスを用いた腫瘍溶解性ウイルス療法のフェーズI試験.

第75回日本癌学会学術総会 (パシフィコ横浜) 2016.10.7

Yabe R, Usui T, Ohama T, Sato K

Protein Phosphatase Methyesterase-1 (PME-1) is a therapeutic target in melanoma.

The 4th Sapporo Summer Seminar for One Health, Sapporo, Japan

2016年9月21日（口頭発表・査読なし）

矢部滝太郎、臼井達哉、大浜剛、佐藤晃一

PP2A脱メチル化酵素PME-1はメラノーマの治療標的となりうる

蓼科先端モデル動物支援　若手技術講習会　蓼科グランドホテル滝の湯

2016年9月16日（ポスター発表）

藤原信行、臼井達哉、大浜剛、佐藤晃一

PP2AとDAPK3はBeclin 1のリン酸化を調節しオートファジーを制御する

蓼科先端モデル動物支援　若手技術講習会　蓼科グランドホテル滝の湯

2016年9月16日（ポスター発表）

藤原信行、渋谷周作、斉藤達哉、審良静男、臼井達哉、大浜剛、佐藤晃一

胃癌におけるProtein Phosphatase 6の役割の解明

第159回日本獣医学会　日本大学

2016年9月6日（口頭発表）

臼井達哉、櫻井優、川崎秀吉、大浜剛、佐藤晃一

エアーリキッドインターフェイス培養法を用いた三次元マウス肺がんモデルの樹立

第159回日本獣医学会　日本大学

2016年9月6日（口頭発表）

正司麗葉、伊賀瀬雅也、盆子原誠、野口俊助、水野拓也.

レオウイルスによる犬の悪性組織球肉腫細胞株に対する細胞死の誘導.

第159回日本獣医学会学術集会（日本大学） 2016.9.6

伊賀瀬雅也、野口俊介、水野拓也.

犬悪性黒色腫に対して腫瘍溶解性ウイルス療法の抗腫瘍効果を増強するシグナル阻害剤の探索.

第159回日本獣医学会学術集会（日本大学） 2016.9.6

長谷川友紀、正司麗葉、辻可奈子、下山由美子、下川孝子、馬場健司、奥田優、板本和仁、水野拓也.

組織学的形態の異なる肥満細胞腫が皮膚と脾臓に発生した猫の一例

第54回山口県獣医学会、山口県獣医師会 2016.8.21

伊賀瀬雅也、黒河内陽介、藤木範之、原口友也、水野拓也.

腫瘍溶解性ウイルス療法を行った犬メラノーマの一例.

第15回日本獣医がん学会（東京コンベンションホール） 2016.6.25

研究推進体発足以前（2013年〜2016年6月）

学術雑誌への研究論文発表（抜粋）

(* corresponding author)

Fujiwara N, Usui T, Ohama T*, Sato K

Regulation of Beclin 1 Phosphorylation and Autophagy by PP2A and DAPK3.

Journal of Biological Chemistry, jbc.M115.704908, 2016

Haruta M, Shimada M*, Nishiyama A, Johmura Y, Le Tallec B, Debatisse M and Nakanishi M.

Loss of maintenance DNA methylation results in abnormal DNA origin firing during DNA replication.

Biochem Biophys Res Commun., 469: 960-966, 2016

Murata K, Sato S, Haruta M, Goshima T, Chiba Y, Takahashi S, Sharif J, Koseki H, Nakanishi M and Shimada M*

Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.

Biochem Biophys Res Commun., 458: 470-475, 2015

Yabe R, Miura A, Usui T, Mudrak I, Ogris E, Ohama T*, Sato K

Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.

PLoS ONE, 10(12): e0145226, 2015

Shibutani ST, Saitoh T, Nowag H, Münz C, Yoshimori T.

Autophagy and autophagy-related proteins in the immune system.

Nat Immunol. 16(10): 1014-24, 2015.

Usui T, Nijima R, Sakatsume T, Otani K, Kameshima S, Okada M, Yamawaki H.

Eukaryotic elongation factor2 kinase controls proliferation and migration of vascular smooth muscle cells.

Acta Physiol (Oxf). 213:472-480, 2015

Nishikawa S, Konno M, Hamabe A, Hasegawa S, Kano Y, Fukusumi T, Satoh T, Takiguchi S, Mori M, Doki Y, Ishii H.

Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26.

Oncology Letters. 9(5):2361-2367, 2015

Iseri T, Shimizu J, Akiyoshi H, Kusuda K, Hayashi A, Mie K, Izawa T, Kuwamura M, Yamate J, Fujimoto Y, Ohashi F.

Computed tomography and magnetic resonance imaging of thoracic chordoma in a Bengal tiger (Panthera tigris tigris).

J Vet Med Sci. 77: 857-860, 2015

Goshima T#, Shimada M*# (# Both authors contributed equally to this work), Sharif J, Matsuo H, Misaki T, Johmura Y, Murata K, Koseki H and Nakanishi M

Mammal-specific H2A Variant, H2ABbd, Is Involved in Apoptotic Induction via Activation of NF-κB Signaling Pathway

J Biol Chem., 289: 11656-11666, 2014

Johmura Y, Shimada M, Misaki T, Naiki-Ito A, Miyoshi H, Motoyama N, Ohtani N, Hara E, Nakamura M, Morita A, Takahashi S and Nakanishi M.

Necessary and sufficient role for a mitosis skip in senescence induction

Molecular Cell., 55: 73-84, 2014.

Hirokawa T, Shiotani B, Shimada M, Murata K, Johmura Y, Haruta M, Tahara H, Takeyama H and Nakanishi M.

CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double strand break specific G2 checkpoint

Cancer Research., 74: 3880-3889, 2014.

Usui T, Sakatsume T, Nijima R, Otani K, Kazama K, Morita T, Kameshima S, Okada M, Yamawaki H.

Death-associated protein kinase3 mediates vascular structural remodeling via stimulating smooth muscle cell proliferation and migration.

Clin Sci (Lond). 127:539-548. 2014.

Shibutani ST, Yoshimori T.

Autophagosome formation in response to intracellular bacterial invasion.

Cell Microbiol. 16: 1619-1626, 2014.

Shimizu K, Mizuno T, Shinga J, Asakura M, Kakimi K, Ishii Y, Masuda K, Maeda T, Sugahara H, Sato Y, Matsushita H, Nishida K, Hanada K, Dorrie J, Schaft N, Bickham K, Koike H, Ando T, Nagai R, Fujii S.

Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.

Cancer Res. 73(1):62-73, 201

Hwang CC, Umeki S, Kubo M, Hayashi T, Shimoda H, Mochizuki M, Maeda K, Baba K, Hiraoka H, Coffey M, Okuda M, Mizuno T*.

Oncolytic reovirus in canine mast cell tumor.

PLoS One. 8(9):e73555, 2013

国際学会又は全国大会での発表（抜粋）

大浜剛

消化管の炎症・癌におけるタンパク質脱リン酸化酵素の役割

第89回日本薬理学会, 2016年3月9日, パシフィコ横浜（シンポジウム）

Usui T

「三次元培養法による肺がんオルガノイド作製」第94回Stanford University LSJ (Life science in Japanese)セミナー, U.S.A, 2015（研究発表）

大浜剛

Type2A Protein PhosphataseによるBeclin 1プラットフォーム機能の制御機構

BMB2015 2015年12月4日, 神戸ポートアイランド（ワークショップ）

Hwang CC, Igase M. Mizuno T.

Oncolytic reovirus in canine mast cell tumor and lymphoma, Asian Meeting of Animal Medicine Specialist 2015

2015.11 Kuala Lumpur（研究発表）

Yabe R, Miura A, Sato K, Ohama T

Protein Phosphatase Methyl Esterase PME-1 protects Protein Phosphatase 2A from ubiquitin/proteasome degradation Europhosphatase 2015

2015年6月24-29日, Turku, Finland（研究発表）

井芹俊恵

気道確保と呼吸管理

第90回獣医麻酔外科学会, 2015.6, 大宮ソニックシティ（招待講演）

水野拓也

未来志向型獣医療　臨床応用が期待される第4の犬のがん治療

第11回日本獣医内科学アカデミー学術集会, 2015.2 , パシフィコ横浜（招待講演）

西川晋平、波多豪、今野雅允、西田尚弘、小関準、川本弘一、佐藤太郎、滝口修司、山本浩文、森正樹、土岐祐一郎、石井秀始

CD26およびCD44をマーカーとした胃癌幹細胞の同定

第73回日本癌学会学術総会, 2014.9, 神奈川県横浜市（研究発表)

Shibutani S, Imai K, Kusaba T, Yoshimori T

Recruitment of Atg Proteins to Selective Autophagy Substrates.

Gordon Research Conference (Autophagy in Stress, Development & Disease), 2014.3, Lucca, Italy（研究発表）

島田緑, 中西真 (2014). New roles of H2AX in DNA damage response and genome stability. 第37回分子生物学会年会ワークショップ, 2014.11, 神奈川県横浜市（招待講演）

島田緑, 中西真 (2014). Mammalian-specific H2A variant, H2A.Bbd, is involved in apoptotic induction via activation of NF-kB signialin pathway. 第66回日本細胞生物学会大会, 2014.6, 奈良県奈良市（招待講演）

Nishikawa S, Ishii H, Konno M, Haraguchi N, Kano Y, Hamabe A, Hasegawa S, Ogawa H, Fukusumi T, Ohta K, Kudo T, Sakai D, Takiguchi S, Satoh T, Mori M, Doki Y

Novel cancer stem cell marker for gastric cancer distinguishes cancer stem cells in CD44 negative population.

Cold Spring Harbor Laboratory meeting, Harnessing Immunity to Prevent and Treat Disease, 2013.11 NY, USA（研究発表）